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https://open.uns.ac.rs/handle/123456789/9485
Title: | Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems | Authors: | Antov, Mirjana Jugović, Branimir Gvozdenović, Milica Knežević Jugović, Zorica |
Issue Date: | 1-Dec-2012 | Publisher: | Novi Sad: University of Novi Sad, Faculty of Technology, Novi Sad | Journal: | Acta Periodica Technologica | Abstract: | This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase. | URI: | https://open.uns.ac.rs/handle/123456789/9485 | ISSN: | 14507188 | DOI: | 10.2298/APT1243151A |
Appears in Collections: | TF Publikacije/Publications |
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