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Title: | Analytical methods for biotin determination | Authors: | Došenović I. Lončar E. Došenović M. |
Issue Date: | 1-Jan-2012 | Journal: | CEFood 2012 - Proceedings of 6th Central European Congress on Food | Abstract: | This paper gives a rewiew of analytical methods for biotin determination in various mediums. Contemporary analytical methods provide fast biotin determination with low detection limits. Results of quantitative determination of biotin in sugar beet molasses obtained by various methods are presented.The following methods were applied: HPLC (High Pressure Liquid Chromatography), TLC (Thin Layer Chromatography), microbiological method by Danielsen and Eriksen (1968) for biotin activity determination in the raw materials for fermentation with active microorganism Saccharomyces cerevisiae and method for the microbiological analysis of selected nutrients given in AOAC International (1996) with active microorganism Lactobacillus plantarum. Microbiological method by Danielsen and Eriksen (1968) with active microorganism Saccharomyces cerevisiae for biotin determination in sugar beet molasses came out accurate, precise, with the detection limit of 0.0100 μg/cm3 or 10 ppm, because it is the lowest concentacion of biotin which still shows linear response and fits into linear dependence of biotin concentrations - growth area of the yeast. For these reasons this method was accepted as the standard method for determination of biotin in sugar beet molasses by the Institute for Standardization of Serbia. | URI: | https://open.uns.ac.rs/handle/123456789/5688 |
Appears in Collections: | TF Publikacije/Publications |
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