Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/29318
DC Field | Value | Language |
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dc.contributor.author | Rat Milica | - |
dc.contributor.author | Jovanović Živko | - |
dc.contributor.author | Stanisavljević Nemanja | - |
dc.contributor.author | Radak Boris | - |
dc.contributor.author | Bokić Bojana | - |
dc.contributor.author | Radović Svetlana | - |
dc.contributor.author | Anačkov Goran | - |
dc.date.accessioned | 2020-12-14T16:34:46Z | - |
dc.date.available | 2020-12-14T16:34:46Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1821-2158 | - |
dc.identifier.issn | 1821-2638 | - |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/29318 | - |
dc.description.abstract | We report an efficient, simple and cost-effective protocol for the isolation of genomic DNA from Ornithogalum species. Our modification of the standard CTAB protocol includes two polyphenol adsorbents (insoluble PVPP and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and addition of phenol to remove proteins and other contaminants. DNA yield obtained with our protocol was 223 and 312 μg DNA g-1 of dry leaf tissue. The absorbance ratio 260/280 nm was 1.879 (O. refractum) and 1.753 (O. sibthorpii), and the absorbance ratio 260/230 nm was 1.779 (O. refractum) and 1.545 (O. sibthorpii), revealing lack of contamination. PCR amplifications of one nuclear marker (26S rDNA) indicated that this DNA isolation protocol may be used for Ornithogalum plants containing many interfering compounds for further analyses in population genetics and phylogeographic studies. © 2014 Institute of Botany and Botanical Garden Jevremovac, Belgrade. | - |
dc.language.iso | en | - |
dc.relation.ispartof | Botanica Serbica | - |
dc.source | CRIS UNS | - |
dc.source.uri | http://cris.uns.ac.rs | - |
dc.title | A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales) | - |
dc.type | Journal/Magazine Article | - |
dc.identifier.scopus | 2-s2.0-84900562315 | - |
dc.identifier.url | https://www.cris.uns.ac.rs/record.jsf?recordId=87289&source=BEOPEN&language=en | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84900562315 | - |
dc.relation.lastpage | 190 | - |
dc.relation.firstpage | 185 | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 38 | - |
dc.identifier.externalcrisreference | (BISIS)87289 | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Departman za biologiju i ekologiju | - |
crisitem.author.dept | Departman za inženjerstvo zaštite životne sredine i zaštite na radu | - |
crisitem.author.dept | Departman za biologiju i ekologiju | - |
crisitem.author.dept | Departman za biologiju i ekologiju | - |
crisitem.author.dept | Departman za biologiju i ekologiju | - |
crisitem.author.parentorg | Prirodno-matematički fakultet | - |
crisitem.author.parentorg | Fakultet tehničkih nauka | - |
crisitem.author.parentorg | Prirodno-matematički fakultet | - |
crisitem.author.parentorg | Prirodno-matematički fakultet | - |
crisitem.author.parentorg | Prirodno-matematički fakultet | - |
Appears in Collections: | PMF Publikacije/Publications |
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