Correlation between retention constants obtained in reversed-phase liquid chromatography and partition coefficients of some benzimidazole derivatives

Abstract

Several calculation procedures for log P values based on the fragmental and atomic contributions are compared with experimental reversed-phase liquid chromatography (RPLC) retention constants of benzimidazole derivatives. The RPLC experiments were performed on HPLC comerrcially available LiChrosorb RP(-18 column with binary solvent mixtures of methanol-phosphate buffer (pH 7) as mobile phase. Retention constant log k0 was determined by the extrapolation method. Good correlaton was found between the retention constants log k0 and log P, as well as m and log P of the compounds investigated.