Određivanje rezidua metabolita nitrofurana u životinjskoj plazmi primenom ultravisokopritisne tečne hromatografije sa masenom spektrometrijom

Abstract

<p style="text-align: justify; ">Izvedeni su razvoj i validacija UHPLC-MS/MS metode za određivanje četiri&nbsp;metabolita nitrofurana u životinjskoj plazmi. Metoda se predlaže za preventivni&nbsp;monitoring stoke za klanje. Validacija je izvedena u opsegu od 0.2 do 5 &mu;g kg-1&nbsp;primenom fortifikovane plazme životinja. CC i CC&beta; vrednosti su određene po&nbsp;zahtevima EU. Metoda je primenjena na preko 200 rutinskih uzoraka, kao i na&nbsp;uzorcima plazme životinja iz Republike Srbije. Analiza metabolita NF u krvnoj&nbsp;plazmi životinja se predlaže za preventivnu identifikaciju kontaminacije&nbsp;nitrofuranima.</p>

<p> An UPLC-MS/MS has been developed and validated to determine residues of<br /> four NF metabolites in animal plasma. The method is proposed as a tool for the preslaughter<br /> monitoring of animal herds. The method has been validated from 0.2-5 &mu;g<br /> kg-1 using fortified animal plasma. CC and CC&beta; were determined according to EU<br /> requirements. The method has been comprehensively evaluated through application<br /> to incurred plasma and over 200 routine samples and samples imported from Republic<br /> of Serbia. Confirmatory testing of NF metabolites in plasma taken from livestock is<br /> suggested as a preventative and screening method that can provide early identification<br /> of contamination pre-slaughter.</p>