Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/15231
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gordana Bogdanović | en_US |
dc.contributor.author | Vladimir Jurišić | en_US |
dc.contributor.author | Nada Kraguljac | en_US |
dc.contributor.author | Jasminka Mrđanović | en_US |
dc.contributor.author | Dimitar Jakimov | en_US |
dc.contributor.author | Koviljka Krtolica | en_US |
dc.contributor.author | Milena Krajnović | en_US |
dc.contributor.author | Zvonko Magić | en_US |
dc.contributor.author | Bratislav Stojiljković | en_US |
dc.contributor.author | Ljiljana Andrijević | en_US |
dc.contributor.author | Tatjana Srdić | en_US |
dc.contributor.author | Mirjana Baltić | en_US |
dc.contributor.author | Stevan Popović | en_US |
dc.date.accessioned | 2020-03-03T14:59:08Z | - |
dc.date.available | 2020-03-03T14:59:08Z | - |
dc.date.issued | 2007-01-01 | - |
dc.identifier.issn | 1452126 | en_US |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/15231 | - |
dc.description.abstract | We report on characteristics of the first human cell line, PC-MDS, derived from a bone marrow of a patient with therapy-related myelodysplastic syndrome (t-MDS) who had no overt post-MDS leukemia. Classic cytology analyses, immunophenotyping, cytogenetic and molecular genetic procedures were used for characterization of the cell line. PC-MDS cells are positive for the expression of CD13, CD15, CD30, CD33, and CD45 antigen. Positive cytochemical staining and immunophenotype analyses indicated that PC-MDS cells have some characteristics of the early myeloid precursor cell. The karyotype analysis of PC-MDS cell line revealed various numerical and structural changes including those typically associated with t-MDS: del(5)(q13)[7], der(5)t(5;11)(p11;q11)[13], -7[6], del(7)(q31)[2], +20[3], -20[4]. Evaluation of methylation status in a promoter region of p15, p16 and MGMT genes showed biallelic hypermethylation pattern of 5′ promoter region only in MGMT gene. PC-MDS is the first t-MDS derived cell line, and based on its immunological, cytogenetic and molecular characterization could be a new tool in evaluation of complex biology of MDS and a model for methylation studies. © 2007 Elsevier Ltd. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Leukemia Research | en_US |
dc.subject | human cell line | en_US |
dc.subject | cytogenetic | en_US |
dc.subject | immunophenotype | en_US |
dc.subject | methylation status | en_US |
dc.subject | Ig and TCR gene rearrangements | en_US |
dc.title | Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.doi | 10.1016/j.leukres.2007.01.012 | - |
dc.identifier.pmid | 31 | - |
dc.identifier.scopus | 2-s2.0-34249939084 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/34249939084 | - |
dc.description.version | Published | en_US |
dc.relation.lastpage | 1105 | en_US |
dc.relation.firstpage | 1097 | en_US |
dc.relation.issue | 8 | en_US |
dc.relation.volume | 31 | en_US |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | Medicinski fakultet, Katedra za biohemiju | - |
crisitem.author.orcid | 0000-0003-0676-7985 | - |
crisitem.author.parentorg | Medicinski fakultet | - |
Appears in Collections: | MDF Publikacije/Publications |
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