Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/14588
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leskovac V. | en |
dc.contributor.author | Peričin D. | en |
dc.date.accessioned | 2020-03-03T14:56:40Z | - |
dc.date.available | 2020-03-03T14:56:40Z | - |
dc.date.issued | 1978-03-30 | en |
dc.identifier.issn | 0006291X | en |
dc.identifier.uri | https://open.uns.ac.rs/handle/123456789/14588 | - |
dc.description.abstract | Commercial lyophilized preparations of yeast alcohol dehydrogenase from Boehringer G.m.b.H. (Mannheim, Germany) bind 2 mols of reduced coenzyme/144000 g of enzyme (1). After the purification by a DEAE-Sephadex column chromatography, the coenzyme binding capacity is raised to 4 mols of NADH/mol of enzyme. Commercial preparations and ionexchange-purified preparations are homogeneous on the ionexchange column chromatography and the disc gel electrophoresis, after reduction with thioglycolic acid. Ionexchange chromatography does not increase the -SH titer, zinc content and the specific activity of enzyme. It is suggested that ionexchange chromatography raises the NADH-binding capacity by removing some impurities present in commercial enzyme preparations. © 1978. | en |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en |
dc.title | Coenzyme binding capacity of yeast alcohol dehydrogenase | en |
dc.type | Journal/Magazine Article | en |
dc.identifier.doi | 10.1016/0006-291X(78)91550-4 | en |
dc.identifier.pmid | 81 | en |
dc.identifier.scopus | 2-s2.0-0017888927 | en |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/0017888927 | en |
dc.relation.lastpage | 426 | en |
dc.relation.firstpage | 422 | en |
dc.relation.issue | 2 | en |
dc.relation.volume | 81 | en |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | TF Publikacije/Publications |
SCOPUSTM
Citations
2
checked on May 10, 2024
Page view(s)
11
Last Week
6
6
Last month
0
0
checked on May 10, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.