Please use this identifier to cite or link to this item:
https://open.uns.ac.rs/handle/123456789/13938
Title: | Investigation of isolation conditions and ion-exchange purification of protein coagulation components from common bean seed | Authors: | Antov, Mirjana Šćiban, Marina Adamović, Slavica Klašnja, Mile |
Issue Date: | 1-Dec-2007 | Publisher: | Novi Sad: University of Novi Sad, Faculty of Technology, Novi Sad | Journal: | Acta Periodica Technologica | Abstract: | Investigation of an extraction procedure of protein coagulanty from common bean seed regarding concentration of NaCl and pH was performed High values of protein concentration and coagulation activity in crude extract (9.19 g/l and 23.9%, respectively) were obtained when the extraction was performed using 0.5 mol/l NaCl and water as solvent, which represents an advantage for economic and environmental reasons. Crude extract of common bean seed was purified by precipitation at two different percentages of (NH4)2SO4 saturation, followed by batch ion-exchange chromatography. The highest obtained coagulation activity, 45%, was determined infraction that was eluated at 1.75 mol/l NaCl from resin loaded with proteins precipitated upon 80-100% (NH4)2SO4 saturation. High values of coagulation activity showed by some eluates suggest their application as natural coagulantfor water purification. | URI: | https://open.uns.ac.rs/handle/123456789/13938 | ISSN: | 14507188 | DOI: | 10.2298/APT0738003A |
Appears in Collections: | TF Publikacije/Publications |
Show full item record
SCOPUSTM
Citations
11
checked on May 10, 2024
Page view(s)
40
Last Week
17
17
Last month
0
0
checked on May 10, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.