Alterations in jejunal morphology and serotonin-containing enteroendocrine cells in peripubertal male rats associated with subchronic atrazine exposure

Abstract

The effect of atrazine on jejunum was investigated by histopathological examination and quantification analysis related to the morphological parameters of the jejunum. The experiment was performed on male Wistar rats from postnatal day 23-51. Atrazine was administered by gavage daily to one group of rats at 50. mg/kg of body weight (bw) dose, to the second at 200. mg/kg. bw while the third group was the control. At the end of the experiments after 28 days of treatment, tissue samples were routinely processed and stained with haematoxylin eosin. Additionally, the histochemical staining with periodic acid Schiff-alcian blue was used to demonstrate goblet cells and the immunohistochemistry protocol for serotonin-containing enteroendocrine cells. A significant decrease in the height of epithelial cells covering the intestinal villi, the villus height to total mucosa thickness ratio and the villus height to crypt depth ratio was found at both doses of atrazine. The crypt depth, total mucosa thickness and the thickness of tunica muscularis were significantly increased by the high dose of atrazine only. The number of serotonin-positive endocrine cells significantly decreased in rats treated with the low dose of atrazine. Overall, these results suggest the alterations in intestinal absorption and support the role of atrazine as an endocrine-disrupting compound. © 2011 Elsevier Inc.