Study of Support-Solvent Effects in Thin-Layer Chromatography on Cellulose and Starch

Abstract

The retention behavior of various classes of homologous compounds has been studied in thin-layer chromatography on cellulose and starch. The effects of the developing solvent composition and of the number of methylene groups in the solute molecule have been discussed using the retention constant, RThe results obtained indicate that the particular classes of homologous compounds studied are separated from one another only be virtue of their functional groups and that the compositions of the stationary and the mobile phases on both cellulose and starch are the same. It was also observed that the polarity differences between the mobile and the stationary phase proved to be changed by varying the molar proportions of the developing solvent components. Therefore, depending on their properties, some classes of solutes examined are separated on “normal” phases and the others on “reversed” phases. © 1982, Taylor & Francis Group, LLC. All rights reserved.