Separation of fat-soluble vitamins by thin-layer chromatography

Abstract

This paper is dealing with the evaluation of the method for qualitative analyses of fat soluble vitamins by thin-layer chromatography on impregnated starch, cellulose and talc thin layers. Impregnation was carried out with paraffin oil. Complete analyses could be obtained on two plates with two different solvents. Vitamins K3, K4 and K5 were separated with solvent I (water-dioxane-acetone-formaldehyde (85:20:15:25)) whereas the other remain on the start position. Vitamins A-acetate, A-palmitate, K1, DL-alpha-tocopherol, DL-alpha-tocopherol-acetate, D2, D3 were separated with solvent II (acetone-concentrated acetic acid (30:20)) while K3, K4 and K5 migrate with front. Quick and simple procedures for the preparation of the plates with an impregnated thin layer is given. © 1976 Friedr. Vieweg & Sohn, Verlagschesellschaft mbH.